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. 2016 Aug 1;172(1):244–253. doi: 10.1104/pp.16.00774

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© 2016 American Society of Plant Biologists. All rights reserved.

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Figure 3. — Complementation of the mat3 mutant and subcellular localization of MAT3. A, Representative wild-type (WT), mat3, and complemented immature siliques and mature siliques after clearing. Bars = 1 mm. B, Subcellular localization of MAT3 in a quartet, in which two of the four pollen grains are transgenic. Images (from left to right) show the 4′,6-diamino-2-phenylindole (DAPI) channel, GFP channel, bright field, and merged. C, Pollen tube lengths of qrt1/qrt1;mat3/mat3, qrt1/qrt1, and mat3/mat3 carrying pMAT3::MAT3-GFP measured after 6 h of germination. Data are means ± se of three experiments. For each independent replicate, 100 to 150 pollen tubes were analyzed.