Figure 10.

Association of AGO1 with chromatin in wild-type, hyl1-2, and cpl1-7 mutant plants monitored under standard and salt stress conditions. A, Association of AGO1 with the MIIR161 gene. B, Association of AGO1 with the MIR173 gene. ChIP was performed using antibodies against AGO1. For the experiment, primers amplifying pre-miRNA coding region were used. For wild-type plants, ChIP with and without RNase A was conducted. The values presented in the charts are the mean ± sd (n = 3). * t test P value ≤ 0.05; ** t test P value ≤ 0.01.