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. 2016 Jul 5;172(1):575–588. doi: 10.1104/pp.16.00379

Figure 1.

Figure 1.

OsERF71 overexpression in rice enhances drought resistance. A, RNA gel-blot analyses showing transcriptional expression of OsERF71 in response to various stress conditions. An OsDIP1 gene probe was used as a positive control for abiotic stresses and the OsRbcS gene as a negative control. Ribosomal RNAs (rRNAs) were used as an internal control to determine equal loading of total RNA. B, RNA gel-blot analyses showing the expression levels and patterns of OsERF71 transcript accumulation in three independent homozygous lines of GOS2::OsERF71 (lines 20, 34, and 49) and RCc3:OsERF71 (lines 12, 34, and 49) plants. Ribosomal RNAs were used as an internal control to determine equal loading of total RNA. C, Drought resistance of GOS2::OsERF71 and RCc3:OsERF71 transgenic rice plants at the vegetative stage. Three independent homozygous GOS2::OsERF71 and RCc3:OsERF71 transgenic lines and NT control plants were grown in soil for 1 month and exposed to drought for 5 d, followed by rewatering. Numbers on the images indicate the duration over which drought was imposed and rewatering was applied. D, Survival rate at 15 d after rewatering. Values represent means + sd of three repeated tests. Each test was used with 38 to 40 plants for each transgenic line and 115 to 128 plants for NT. Asterisks indicate significant differences compared with NT (P < 0.05 by Student’s t test).