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. 2016 Jul 5;172(1):575–588. doi: 10.1104/pp.16.00379

Figure 3.

Figure 3.

OsERF71 spatiotemporal expression and subcellular expression patterns of GOS2::GFP and RCc3::GFP. A, qRT-PCR analysis of OsERF71 expression in rice tissues of 10- and 30-d-old NT rice plants at the vegetative developmental stage and before heading (BH) and after heading (AH) in NT rice plants at the reproductive stage. F, Flower; FL, flag leaf; L, leaf blade; LS, leaf sheath; R, root. UBIQUITIN1 expression was used as an internal control. Data are shown as means ± sd of three biological and two technical replicates. B, qRT-PCR analysis of OsERF71 expression in 2-week-old GOS2::OsERF71 (lines 20, 34, and 49), RCc3:OsERF71 (lines 12, 34, and 49), and NT roots. UBIQUITIN1 expression was used as an internal control. Data are shown as means + sd of three biological and two technical replicates. C to H, Confocal microscopy images of 2-week-old OsERF71::GFP roots. C to E, Root apex in bright-field image (C), propidium iodide (PI)-stained image (D), and GFP expression (E). F and G, Cross sections of a root in the elongation zone, 3 mm above the root tip, in PI-stained image (F) and GFP merged image (G). Insets in F and G are whole images. H, Cross section of a root in the maturation zone, 1 cm above the apical tips. Bars in C to H = 100 μm. I, Schematic diagram representing the organization of an NT root in the elongation zone in transverse section. J to N, Confocal microscopy images of 2-week-old GOS2::GFP roots. J and K, Root apex in bright-field image (J) and GFP expression (K). L to N, Cross sections of a root in the elongation zone, 3 mm above the root tip, in PI-stained image (L), GFP expression (M), and merged image (N). Inset in N is the whole image. Bars = 1 mm in J and K and 100 μm in L to N. O to S, Confocal microscopy images of 2-week-old RCc3::GFP roots. O and P, Root apex in bright-field image (O) and GFP expression (P). Q to S, Cross sections of a root in the elongation zone, 3 mm above the root tip, in PI-stained image (Q), GFP expression (R), and merged image (S). Bars = 1 mm in O and P and 100 μm in Q to S. Arrowheads, Endodermis; arrows, pericycle; ic, inner cortex.