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. 2016 Jul 5;172(1):575–588. doi: 10.1104/pp.16.00379

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Figure 6. — ChIP-qPCR for OsERF71 interacting with the promoters of the selected lignin biosynthesis genes. Two-week-old RCc3::OsERF71-myc and NT roots were used in ChIP-qPCR experiments with an anti-myc antibody. Left, ChIP-qPCR analysis showing the promoters of four lignin biosynthesis genes with four PCR-amplified regions (P1–P4). Right, ChIP-qPCR data indicate each PCR amplification region of each gene. The fold enrichment was normalized to ChIP-qPCR data of wild-type (WT) roots. Data are shown as means + sd of three technical replicates. The asterisk indicates a significant difference based on a 95% confidence interval. DRE, G/ACCGAC; CRT, GTCGAC; and GCC core, GCCGCC.