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. 2016 Jul 2;172(1):141–153. doi: 10.1104/pp.16.00460

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© 2016 American Society of Plant Biologists. All rights reserved.

http://www.aspb.org/publications/aspb-journals/open-articles.

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Figure 5. — Overexpression of the N-end-rule-insensitive Δ13RAP2.12 transcription factor under the control of the 35S promoter leads to severe changes in metabolite profiles. Metabolite levels from whole rosettes of 5-week-old wild-type, RAP2.12, and Δ13RAP2.12-overexpressing plants sampled after 16 h incubation in normoxia or hypoxia in the absence of sugars in the dark are visualized using the following VANTED diagrams: A, Carbohydrate metabolism and pentose P-pathway; B, glycolysis, fermentation, Ser, and shikimate pathways; and C, tricarboxylic acid cycle, Asp, and Glu metabolism. Results are means ± se (n = 8–10 biological replicates) and are expressed as arbitrary values; the scaling of the y axis was therefore omitted. Values of the different genotypes that significantly differ from each other within one oxygen treatment are indicated by different letters (according to one-way ANOVA test, P < 0.05). Metabolite ratios are shown in boxes with gray background color. In Supplemental Table S1, the complete metabolite profile is shown together with a statistical analysis of the data. Growth of plants and rosette stage analyzed was similar as in Fig. 2. WT, Wild type.

Figure 5. — Overexpression of the N-end-rule-insensitive Δ13RAP2.12 transcription factor under the control of the 35S promoter leads to severe changes in metabolite profiles. Metabolite levels from whole rosettes of 5-week-old wild-type, RAP2.12, and Δ13RAP2.12-overexpressing plants sampled after 16 h incubation in normoxia or hypoxia in the absence of sugars in the dark are visualized using the following VANTED diagrams: A, Carbohydrate metabolism and pentose P-pathway; B, glycolysis, fermentation, Ser, and shikimate pathways; and C, tricarboxylic acid cycle, Asp, and Glu metabolism. Results are means ± se (n = 8–10 biological replicates) and are expressed as arbitrary values; the scaling of the y axis was therefore omitted. Values of the different genotypes that significantly differ from each other within one oxygen treatment are indicated by different letters (according to one-way ANOVA test, P < 0.05). Metabolite ratios are shown in boxes with gray background color. In Supplemental Table S1, the complete metabolite profile is shown together with a statistical analysis of the data. Growth of plants and rosette stage analyzed was similar as in Fig. 2. WT, Wild type.