Figure 7.

Overexpression of the N-end-rule-insensitive Δ13RAP2.12 transcription factor under the control of the 35S promoter leads to decreased anoxic survival rates, which can be prevented by external feeding of 30 mm Suc. A, Seedlings of 1-week-old wild-type, RAP2.12, and Δ13RAP2.12-overexpressing plants grown on MS medium with (+) or without (−) 30 mm Suc were exposed to anoxic conditions for 6.5 and 8 h, respectively, before survival rates were measured 1 week later. B, To put the data into context, starch levels were measured in the shoots of the seedlings at the time point of transfer to anoxic conditions (2 h into the photoperiod). Results are means ± se (n = 10 different sets of plants for survival rates or 5–6 for starch levels). Values of the different genotypes that significantly differ from each other are indicated by different letters (according to one-way ANOVA test, P < 0.05). WT, Wild type.