Figure 3.

Effect of cytokinin on the steady-state levels of the metabolites of the tetrapyrrole biosynthesis pathway. A, Simplified scheme of the chlorophyll biosynthesis pathway. Names of genes and proteins analyzed in this study are given at the respective metabolic step. B to F, Levels of intermediate metabolites of chlorophyll biosynthesis at different time points following treatment with cytokinin. Wild-type (WT) and ahk2 ahk3 seedlings were grown for 3 d in dark on medium (MS, no Suc) with or without 1 µM BA prior to analysis. Letters indicate significant differences to the wild type (control conditions) (a, ahk2 ahk3, control conditions; b, wild type, BA; c, ahk2 ahk3, BA) and to the wild type (BA) (d, ahk2 ahk3, BA) (P < 0.05); error bars represent se (n = 5).