Figure 6.

Microfluidic chambers are amenable to confocal microscopy. A, YFP targeted to the Golgi reveals a tip-focused accumulation of Golgi dictyosomes. mCherry targeted to the mitochondria. GFP targeted to the peroxisomes. GFP-GUS fusion targeted to the nucleus. Scale bar, 25 µm. B, A cell division event observed in a rhizoid emerging from the base of a gametophore. Moss tissue expressing Myo8A-GFP (green) and mCherry-tubulin (red) was imaged on a scanning confocal microscope. Images are maximum intensity projection of z-stacks from a time-series acquisition. Scale bar, 10 µm. See Supplemental Movie S8.