Figure 5.

A gain-of-function mutation (gai-1) of GAI represses the earlier flowering phenotype of CO-overexpressing plants. A and C, Flowering phenotypes of various gene types under LDs. All plants are in Col-0 background. Error bars indicate the SD of total leaf number; statistics by Student’s t test; *P < 0.01. B and D, qRT-PCR analysis of FT expression levels in various plants. The ACTIN2 gene was used as an internal control. Total RNA was extracted from 10-d-old plants at ZT 16 grown under LD. Error bars indicate sd from three independent RNA extracts; statistics by Student’s t test; *P < 0.05.