Figure 6.

Analysis of DELLA transcript and protein accumulation under LD photoperiod. A, qRT-PCR of DELLA and CO relative to IPP2 under LDs. Total RNA was extracted from 10-d-old Ler seedings. Error bars indicate sd from three independent RNA extracts. B, Western-blot analysis of GFP-RGA protein accumulation. The blots from seedlings of 10-d-old wild type (Ler) or homozygous proRGA:GFP-RGA transgenic plants. An anti-GFP mouse antibody (Sigma-Aldrich) was used to detect GFP-RGA. β-Tubulin was used as an internal control, and GFP-RGA/β-tubulin ratios were calculated and shown. Western-blot experiments were repeated at least three times, and similar results were obtained. Time is expressed as hours from dawn.