Figure 7. Klf5 is required for satellite cell differentiation.
(A) Isolated SCs from Klf5 knockout mice (Pax7CE/+;Klf5flox/flox) were cultured in the presence or absence of 4OH-Tmx in growth medium for 2 days. Purified protein derived from primary myotubes differentiated for 3 days were analyzed by western blotting. Representative blots from three individual experiments are shown. (B) Relative mRNA expression of Myog Myl4 and Myom2 in SCs differentiated for 3 days. Data represent means ± SEM (**p<0.01). Representative data from three individual experiments are shown.