The costimulatory molecules CD86/CD80 are induced less in NOD DCs. NOD or B6 mice were treated with CpG i.v., and 12 h post-treatment, cells from the indicated lymphoid organs (spleen, pLNs, and sLNs) were stained for costimulatory molecule expression and DC subset markers (CD8+ cDC, CD11b+ cDC, and pDC subsets). CD86 expression (A) and CD80 expression (B) in DC subsets. Data presented as geometric mean fluorescence intensity (MFI). Data were pooled from three independent experiments. NOD and B6 strains after CpG stimulation were compared by Student t test. *p < 0.05, **p < 0.01, ***p < 0.001.