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. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: J Immunol. 2016 Jan 29;196(5):2031–2040. doi: 10.4049/jimmunol.1501239

FIGURE 4.

FIGURE 4

NOD cDCs express higher CD40, and NOD moDCs are the source of increased IL-12 and TNF. NOD or B6 mice were treated with CpG i.v., and 12 h posttreatment, cells from the indicated lymphoid organs (spleen, pLNs, and sLNs) were stained for CD40, TNFR superfamily member, and DC subset markers (CD8+ cDC, CD11b+ cDC, and pDC subsets) (A). Spleen CD11c+ DCs were bead-enriched from NOD and B6 mice and stimulated with the indicated concentration of CpG in vitro. IL-12p70 and TNF-α were measured in the culture supernatant by ELISA 12 h post-CpG stimulation (B). Six hours post-CpG injection, serum samples were collected for IL-12p70 and TNF-α analysis (B). Intracellular cytokine levels (IL-12p40 and TNF-α) were measured in CD8+ cDC, CD11b+ cDC, and moDC flow cytometry 12 h post-CpG stimulation (C and D). Data were either pooled from two to three independent experiments (A and B) or presented one representative experiment from three repeated experiments (C and D). *p < 0.05, **p < 0.01, ***p < 0.001.