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. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: J Immunol. 2016 Jan 29;196(5):2031–2040. doi: 10.4049/jimmunol.1501239

FIGURE 6.

FIGURE 6

CD86 expression in NOD mice is more dependent on signaling downstream of IFNAR than CD40. (A) BMDCs were cultured with Flt3L, and cultures were blocked with anti-IFNAR Ab for 24 h before stimulation with CpG in vitro. CD86 (left panel) and CD40 (right panel) expression were measured by flow cytometry on cells gated on CD11c+ DCs. (B) CpG was given i.v., and anti-IFNAR Ab was injected i.p. in mice 24 h before i.v. CpG stimulation. Twelve hours post-CpG treatment, spleen cells from NOD and B6g7 mice were gated on CD8+ cDC and CD11b+ cDC subsets, and CD86 and CD40 were measured by flow cytometry. Data were pooled from three independent experiments. Data represent fold change compared with CpG-B6g7. Each dot represents an individual mouse. Values are geometric mean fluorescence intensity (MFI). *p < 0.05, **p < 0.01.