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. Author manuscript; available in PMC: 2017 Oct 21.
Published in final edited form as: ACS Chem Biol. 2016 Sep 12;11(10):2908–2914. doi: 10.1021/acschembio.6b00624

Figure 2.

Figure 2

Bile acid recognition by NAPE-PLD. (A) Kinetics parameters of bile acids and compound analogues (CHAPS, Fusidic acid) against NAPE-PLD, determined at 25 °C using surface plasmon resonance. (B) FastStep kinetics response profiles (and their replicates) for selected compounds. The concentration profile of the association phase was created within the biosensor SensìQ Pioneer using a twofold dilution series, and 100 μM as the highest analyte concentration. Red lines show a global fit to the response data used to extract the binding constants reported in the table (A).