Figure 2. Cellular analysis of Wls and Wnts after DDC diet.
(A) Parenchymal cells (PC), EpCAM+ cells, and CD45+ cells were separated from non-parenchymal cells after liver perfusion. (B) Expression of EpCAM, albumin (Alb), and Wls in PC, CD45+ cells, and EpCAM+ cells is shown by qRT-PCR. (C) Wls, Wnt7A, Wnt7B, and Wnt10A expression increases in EpCAM+ cells after DDC treatment, while expression of Wnt2 decreases after DDC, as shown by qRT-PCR.