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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Hepatology. 2016 Sep 26;64(5):1652–1666. doi: 10.1002/hep.28774

Figure 3. Wnt7B and Wnt10A induce proliferation of cholangiocytes, while Wnt7A increases Sox9 expression in hepatocytes.

Figure 3

(A) Expression of Wnt7A, Wnt7B, and Wnt10A expression in small cholangiocyte (sm-cc) cell cultures after transfection with Wnt-expressing plasmids. (B) A significant increase in proliferation as determined by Wst-8 assay was evident in sm-cc cells expressing Wnt7B or Wnt10A plasmid, but not Wnt7A. (C) Human 293T cells expressing either Wnt7A, Wnt7B, or Wnt10A were co-cultured with either mouse AML12 cells or primary mouse hepatocytes. (D) Wnt mRNA expression in 293T cells after transfection. (E) Mouse Sox9 and GAPDH mRNA expression in total cell lysates demonstrates the specificity of these primers in identifying gene expression from mouse hepatocytes only. (F) 293T cells expressing Wnt7A induce Sox9 expression in co-cultured AML12 cells and primary hepatocytes, as measured by qRT-PCR. * p < 0.05, ** p < 0.01.