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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Hepatology. 2016 Aug 23;64(5):1559–1576. doi: 10.1002/hep.28747

Figure 3. NgBR deficiency increases hepatic lipogenesis.

Figure 3

(A) Protein levels of NgBR, FASN, and mature SREBP-1c (nSREBP-1c) in shNSi and shNgBRi cells were determined by western blotting. (B) HepG2 cells were transfected with an NgBR overexpression vector at the indicated concentrations for 24 h followed by determination of NgBR, FASN, and nSREBP-1c protein expression by western blotting. (C) Total protein was extracted from the livers of littermate control (NgBR-floxed, no cre) and NgBR LivKO female mice (8 weeks old) that were fed a Western diet for 4 weeks, and protein levels of FASN, precursor of SREBP-1c (pSREBP-1c), nSREBP-1c, and SCD-1 were determined by western blotting. (D) Total RNA was extracted from liver samples of littermate control and NgBR LivKO mice, and expression of genes for FFA and TG biosynthesis, FFA/TG lipolysis and FFA uptake was determined by real-time RT-PCR. *: P<0.05 vs. control (n=4). The sequences of the primers are listed in Table S1.