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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Cell Chem Biol. 2016 Sep 15;23(10):1217–1227. doi: 10.1016/j.chembiol.2016.08.009

Figure 5. Biosynthesis of 2-AA-LPC, PGE2-LPC, 11-HETE-LPC and 15-HETE-LPC in murine lung fibroblasts (MLF) from wild-type (WT) and iPLA2γ-/-(KO) mice stimulated with calcium ionophore.

Figure 5

A , Endogenous 2-AA-LPC production from MLF cells from WT and KO mice stimulated with A23187 (1μM) or vehicle alone at 37°C for 15 min.; B, Endogenous production of PGE2-LPC, 11-HETE-LPC and 15-HETE-LPC in MLF cells from WT and KO mice stimulated with or without (vehicle alone) A23187 calcium ionophore; C, Quantitation of PGE2-LPC, 11-HETE-LPC and 15-HETE-LPC released into the extracellular media of MLF cells from WT and KO mice stimulated with or without (vehicle alone) A23187 calcium ionophore. Data are expressed as mean values ± SEM of six replicates. Statistical analyses were performed using unpaired two-tailed Student t test; P<0.05 was considered significant. *P<0.05 and **P<0.01.