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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Mol Microbiol. 2016 Aug 25;102(3):488–505. doi: 10.1111/mmi.13474

Figure 8.

Figure 8

GlcCer synthesis contributes to the maintenance of neutral lipid levels. A reduced abundance of triacylglycerols and sterols was observed in the gcsA disruptant. An analysis of the neutral lipid content: (A) Triacylglycerol and sterol levels were quantified in WT and ΔsdeA, ΔsmtA, ΔsmtB and ΔgcsA mutants. The results represent the mean value ± SEM, *p < 0.05, (B) For comparison of the TLC of ΔgcsA and WT extracts, lipid extraction was performed with equal weights of mycelia. The total lipids were suspended in chloroform: methanol 2:1, and identical volumes were applied to a silica plate. Neutral lipid separation was carried out using hexane: ethyl ether: acetic acid 6:4:1, and 25 μg of monoacylglycerol, triacylglycerol, sterol, fatty acids and esterified sterol standards was used as a control of the migration pattern. Lipid bands were visualized by Charring reagent staining.