FIG 1.

Overview of LCP proteins in C. glutamicum and their impact on cellular growth. (A) Phylogenetic conservation of lcpA among related species (red arrows). Neighboring conserved genes are colored accordingly. White indicates other, nonconserved proteins of known function, and gray represents hypothetical or uncharacterized proteins. Data were obtained from reference 45. The corresponding figure for lcpB is presented in Fig. S1A in the supplemental material. (B) Domain organization of LcpA and LcpB. (C) Silencing of lcpA expression. Given are relative expression levels of lcpA in different strains and on different media, determined by RT-qPCR. G+I-Mix is a carbon source mixture of 1% (wt/vol) glucose and 1% (wt/vol) myo-inositol (final concentrations). The averages and standard deviations of results for three biological replicates with two technical replicates each are presented. (D) Growth of the lcpA-silencing strain SilG-lcpA on glucose or gluconate as carbon source in comparison to the wild type. Cells were cultivated in complex media with gluconate followed by two consecutive cultivations in CGXII minimal media with the indicated carbon source. The averages and the standard deviations of results for three biological replicates of the second CGXII culture are presented. (E) Impact of lcpA silencing and/or lcpB deletion on growth of C. glutamicum. The same cultivation procedure was used as that described for panel D, with glucose as carbon source.