FIG 4.

LcpA is presumably involved in linking arabinogalactan and peptidoglycan. (A and B) Sugar content in arabinogalactan (A) and remaining sugar content in SDS-purified, ProtK-treated cell walls (B) after extraction of arabinogalactan. *, P < 0.05; **, P < 0.01 (two-sided t test compared to wild type). (C) Mycolic acids were liberated as mycolic acid methyl esters (MAME) from purified cell walls by acidic methanolysis. TLC was run in toluene:acetone (97:3, vol/vol) and developed with phosphomolybdic acid. CS-SilG-lcpA, material from the culture supernatant of SilG-lcpA. Results from three technical replicates of one sample each are presented. The full picture is provided in Fig. S5 in the supplemental material. (D) Relative amounts of MAMEs per milligram of cell wall. *, P < 0.05 (two-sided t test compared to wild type).