FIG 3.

Synthetically misshapen mutant combinations in the O-antigen and ECA pathways. (A) Biosynthetic pathway illustrating how the ECA and O-antigen synthesis pathways compete for Und-PP–GlcNAc. Abbreviations: GlcNAc, N-acetylglucosamine; ManNAcA, N-acetylmannosaminuronic acid; Und-P, undecaprenyl phosphate; Und-PP, undecaprenyl pyrophosphate; ECA, enterobacterial common antigen. (B) Micrographs of cells with the indicated genotypes. Cells were grown and imaged as described in the legend to Fig. 2. Bar, 3 μm. (C) Flow cytometry data from live cells in panel B. Histograms of the forward scatter area from 100,000 events (cells) are shown. The mean cell size for wild-type cells (red graph) is represented by the dashed line and is expressed in arbitrary units (AU). Data are representative of those from two independent experiments. The strains tested were MAJ330 (wild type), MAJ356 (ΔwecB), MAJ346 (ΔwbbJ), MAJ344 (ΔwbbK), MAJ398 (ΔwecB ΔwbbJ), and MAJ397 (ΔwecB ΔwbbK).