FIG 3.

Number of viable P. aeruginosa cells in the planktonic and biofilm populations after the biofilm enrichment assay. After both the first (A) and second (B) 24-h challenge in biofilm-inducing medium, 1 ml of the planktonic culture was collected, serially diluted, and plated to measure the number of CFU. Additionally, after both the first (C) and second (D) 24-h challenge in biofilm-inducing medium, the total biofilm population at the air-liquid interface in each well was isolated using a cell scraper, washed, resuspended in PBS, serially diluted, and plated to measure the number of CFU. Error bars represent standard deviations from three replicates. *, significant difference (P < 0.05, Student's t test) of the specified condition from the equivalent condition without DMS3vir; ns, no significant difference.