Figure 4.

mRNA and Protein Expression in Murine LA

(A) Relative quantity (RQ) of messenger ribonucleic acid (mRNA) of selected ion channels in LA from Pitx2c^+/– and WT mice. Expression levels were measured relative to WT sample 1. Kcnk5 encodes TWIK-related acid-sensitive K⁺ channel 2 (TASK-2), Kcna6 encodes K_v1.6, and Scn5a encodes Na_v1.5. *p < 0.05. (B) Concentration of TASK-2, K_V1.6, and Na_v1.5 proteins relative to calnexin (arbitrary units). Representative immunoblots are displayed below the corresponding dot plot. *p < 0.05. Abbreviations as in Figures 1 and 2.