Polyphosphate accumulation alters M. tuberculosis metabolism. Metabolites altered in the M. tuberculosis
ppk2-deficient and ppx1-deficient mutants relative to the wild-type strain include those belonging to the following categories: phosphate compounds (A), cell wall synthesis pathways (B), NADH metabolism (C), the citric acid (TCA) cycle (D), and arginine metabolism (E). WT, wild-type strain; ppk2, ppk2::Tn mutant; ppx1, ppx1::Tn mutant. The data represent 5 independent samples. *, P < 0.05 compared to the wild type.