FIG 3.

Effect of acto/bezlo or vancomycin on C. difficile burdens in intestinal content and feces of infected animals in rodent CDI models. (A) Levels of C. difficile spores (in CFU/ml, expressed on log₁₀ scale) in intestinal content of individual infected hamsters treated with vehicle (blue circles), with acto/bezlo (red circles), or with vancomycin (green circles). Samples were obtained at predefined time points or at the time of death for animals that did not survive to predefined time points. Since not all animals were sacrificed at exactly the same time on each specified day, the data are plotted at the time (expressed in hours) when the animals were sacrificed. All values are averages of individual values recorded for ileum, colon, and cecum in each animal (see Table S2 in the supplemental material). Numbers in parentheses indicate numbers of animals with burdens below the limit of detection (LOD) (overlapping points) at indicated time points within specified groups (color indicates group per legend above). (B) Per panel A legend but for infected mice. (C) Levels of C. difficile spores (in CFU per milliliter, expressed on log₁₀ scale) in feces of infected hamsters treated with vehicle (Veh; blue circles), with acto/bezlo (red circles), or with vancomycin (Van; green circles). Samples were obtained from the “Survival” arm (see Fig. S1) at predefined time points (days 1, 2, 5, 10, 15, 21, and 28). Averages ± standard deviations are indicated for each time point and treatment. (D) Per panel C but for infected mice. The limit of detection (LOD) for this assay was 17 CFU/ml as indicated by the dotted line.