TABLE 2.
Tm values for C. glabrata FKS1 HS1 and FKS2 HS1
| HS1 variant | Tm (°C) | SD (°C) |
|---|---|---|
| FKS1 | ||
| WT | 58.7 | 0.2 |
| I634V (A1900G) | 62 | 0.2 |
| F625S (T1874C) | 55.2 | 0.2 |
| D632E (T1896A); F625F (C1875T)a | 54.2 | 0.4 |
| S629P (T1885C) | 52.8 | 0.2 |
| D632Y (G1894T); D632H (G1894C) | 51.3 | 0.5 |
| FKS2 | ||
| WT | 61 | 0.2 |
| F659del (del1957-1977) | 62.7 | 0.1 |
| F659V (T1975G) | 59.4 | 0.1 |
| F659S (T1976C) | 58.4 | 0.1 |
| F659L (C1977A) | 57.8 | 0 |
| S663P (T1987C); S663F (C1988T) | 56.4 | 0.2 |
a
Silent mutation can be differentiated from the “true” resistance-associated mutation D632E (T1896A) with the second-tier assay using the F625F-specific MB probe.