FIG 2.
Schematic map of multidrug resistance mosaic region (MRR) in pYD786-1. (A) Evolution of the Tn21-like module in pYD786-1. The integration locations of the integron, IS1326, the IS1353-like element, and the gene cassette array are indicated by vertical arrows. Duplication of the blaCTX-M-2 module is also indicated. DNA fragments shaded with basketwork showed 99% identity to those in the chromosome of Kluyvera ascorbata (accession no. AJ272538.2). The predicted open reading frames (ORFs) and insertion sequences are indicated by bold arrows and annotated above or below the maps, with arrowheads showing the direction of transcription. Putative direct repeat (DR) sequences are given to indicate the boundaries. Paired filled/unfilled squares or circles represent DRs of transposition units. The features shown are not necessarily drawn to scale. (B) Features of In27 and its promoter region in pYD786-1. attI and 59-be of the gene cassette are indicated by a filled oval. The promoters Pc (for the dfrA12 gene) and Pint (for the intI1 gene) are underlined. The position of unique GGG nucleotides in P2 of In2 is indicated. The position of and direction of transcription from the promoters are indicated by vertical and horizontal arrows, respectively.