TABLE 3.
Performance of the direct PCR of the perianal swab compared to the gold standard of PCR of bacteria isolated from chromogenic agar
| Result from direct PCR from swab | No. of swabs with indicated result from PCR of bacteria isolated from chromogenic agara |
|||||||
|---|---|---|---|---|---|---|---|---|
| KPC-pos. | KPC-neg. | CTX-M-pos. | CTX-M-neg. | CMY2-LATCEF-pos. | CMY2-LATCEF-neg. | ACT/MIR-pos. | ACT/MIR-neg. | |
| KPC-pos.b | 11 | 0 | ||||||
| KPC-neg. | 1 | 146 | ||||||
| CTX-M-pos.c | 49 | 5 | ||||||
| CTX-M-neg. | 1 | 103 | ||||||
| CMY2LATCEF-pos.d | 13 | 8 | ||||||
| CMY2LATCEF-neg. | 0 | 137 | ||||||
| ACT/MIR-pos. | 5 | 9 | ||||||
| ACT/MIR-neg. | 1 | 143 | ||||||
a
pos., positive; neg., negative.
b
The sensitivity of the direct PCR from the swab to detect KPC-producing Enterobacteriaceae was 92% (95% confidence interval [CI]: 61% to 100%), and the specificity was 100% (95% CI: 98% to 100%).
c
The sensitivity of the direct PCR from the swab to detect CTX-M-producing Enterobacteriaceae was 98% (95% CI: 89% to 100%), and the specificity was 95% (95% CI: 89% to 98%).
d
The sensitivity of the direct PCR from the swab to detect plasmid-mediated-AmpC-producing Enterobacteriaceae (CMY2-LATCEF or ACT/MIR) was 95% (95% CI: 74% to 100%), and the specificity was 91% (95% CI: 86% to 95%).