Fig. 4.

Regulation of Schwann cell survival by BGJTD treatment. Schwann cells prepared from intact sciatic nerve were treated with DMSO vehicle, taxol, and taxol plus BGJTD. Fixed cells were used for immunostaining with anti-S100β antibody (a) and anti-caspase 3 antibody (b). Cells were also stained with Hoechst33258 to visualize individual nuclei (blue). a Morphological comparison of S100β-stained Schwann cells after different treatments. b Immunofluorescence staining of caspase 3 in cultured Schwann cells. c Quantitative comparison of caspase 3-positive cells in cultured cells after different treatments. A percentage of caspase 3-positive cells from randomly selected microscopic fields (more than 5 fields) were determined from each experiment. *p < 0.05, ***p < 0.001 (One-way ANOVA, number of independent experiments = 4). Scale bars in (a-c): 100 μm