Fig. 7.

Effects of BGJT subgroup decoctions on neurite outgrowth of taxol-treated DRG sensory neurons. Six days after taxol or DMSO vehicle injection in vivo into the sciatic nerve, DRG neurons were cultured in the presence of individual decoctions Ba to Be (0.5 mg/ml) for 48 h and harvested for immunostaining. a Comparison of neurite length among experimental groups. b Representative images of cultured cells after immunofluorescence staining with NF-200 (Green) and GAP-43 (red). Cell nuclei were visualized by nuclear staining with Hoechst 33258 (blue). In (a), neurite length was determined by analyzing more than 20 neurons in 7–10 random microscopic fields, averaged 4 independent experiments, and compared among experimental groups. Error bars in (a) denote standard error of mean (SEM). *P < 0.05, **P < 0.01, ***p < 0.001 (One-way ANOVA, N = 4). Scale bars in (b): 100 μm