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. 2016 Oct 22;16:596. doi: 10.1186/s12879-016-1924-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — ELISA detection of anti-TcdA/B antibody titers. The data are expressed as geometric mean titer (GMT) of TcdA-specific antibody ± standard deviation (SD) of 10 mice per group. The lower limit of detection (1:10) is shown as dotted lines. The experiment was repeated at least twice. a TcdA-specific IgG antibody responses in mouse sera collected at 7 days after each vaccination. b: TcdA-specific IgG1 and IgG2a antibody responses in mouse sera obtained 7 days after the last boost. c TcdB-specific IgG antibody responses in mouse sera collected at 7 days after each vaccination. d TcdB-specific IgG1 and IgG2a antibody responses in mouse sera obtained 7 days after the last boost