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. 2016 Oct 22;16:596. doi: 10.1186/s12879-016-1924-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Survival in vaccinated Balb/C mice following challenge with purified. C. difficile toxins. Balb/C mice (10 mice/group) were challenged i.p with (a) 50 ng TcdA; (b) 25 ng TcdB; or (c) 50 ng TcdA and 25 ng TcdB. 10 days following the second boost DNA immunization, and monitored for survival for 14 days. *, P ≤ 0.05; ***, P ≤ 0.001. Data from two replicate experiments are shown