Table 1.

Phytochemical analysis of MECC by TLC

Phytochemicals	Color	Retention factor (Rf)
		Extract	Standard
Flavonoids	Yellow, orangea	1.02, 0.20	0.41
Phenolics	blue fluroscencea	0.04, 0.10, 0.14, 0.18, 0.47	0.22
Steroids	Purpleb	0.12, 0.13, 0.16	0.34
Glycosides	Dark-violetb	0.94	0.60
Alkaloids	Light pinkb	0.58, 0.83	0.54

R_f values are the mean of two chromatograms

^a Represents the color was observed under the visible or UV light

^b Represents the color was observed after applying the appropriate spray reagent. The standard for flavonoids, phenolics, steroids, glycosides and alkaloids was quercetin, gallic acid, stigmasterol, digoxin, and atropine respectively