Box plots show median and interquartile range of observations and whiskers represent minimum and maximum observations. P values refer to indicated gene expression ratios of fetal brain comparing BTBR and B6 (shown by the dotted line = 1). Fetal brains were collected from pregnant B6 and BTBR mice on E13. In BTBR fetal brains there were significant downregulation of α3 (p<0.0001), α4 (p=0.004), α7 (p=0.006), β2 (p=0.007), and β4 (p=0.001) nAChR mRNA levels compared to B6 fetuses. N= 5 pooled pregnancies per genotype. * indicates p<0.05, † p≤0.01, and ‡ p≤0.001.