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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Ann Biomed Eng. 2016 Apr 22;45(2):360–377. doi: 10.1007/s10439-016-1619-1

FIGURE 4.

FIGURE 4

Circularity of encapsulated HADMSC, HAVIC, and HASSMC stained with calcein-AM at day 1 of culture. Hydrogel precursor solution was made with increasing (A) Irgacure 2959 and (B) VA086 concentration and photocrosslinked with a Lamp at 2 mW/cm2. Average circularity values for day 1 are listed in Table S2. Results of two-way ANOVA are summarized in Table S16 and Table S17. Non-matching letters on the graphs indicate p<0.05 for Tukey HSD post-hoc test and standard error of the mean shown by error bars. At day 1 in culture there was a statistically significant interaction between the effect of cell type and the effect of Irgacure photoinitiator concentration on the circularity of live cells (F=7.5042 p=0.0003, Table S16). At day 1 in culture there was not a statistically significant interaction between the effect of cell type and the effect of VA086 photoinitiator concentration on the circularity of live cells (F=1.8884 p=0.1438, Table S17) but the circularity of cells in VA086 hydrogels does depend on the cell type (p<0.0001).