Figure S6.

L-arginine Upregulates Sirtuin-1, Related to Figure 6

(A) Copy numbers of Sirtuin-1 (SIRT1) as determined by quantitative MS in human naive CD4⁺ T cells activated in normal medium or L-Arg-medium. n = 3 from three donors.

(B) T cell survival experiment. The Sirtuin-1 inhibitor Ex-527 was added at the time point of activation at a concentration of 5 μM. n = 16 from four donors.

(C) T cell survival experiments with clones expressing Cas9 only, or clones devoid of Sirtuin-1. n = 16 from 6 clones. Right panel: western blot of two different Sirtuin-1 knockout clones generated with different sgRNAs. ^∗ unspecific band. For statistical tests, a two-tailed unpaired Student’s t test was used throughout, n.s. non significant; ^∗p < 0.05; ^∗∗p < 0.005; ^∗∗∗p < 0.0005; ^∗∗∗∗p < 0.0001. (B and C) Error bars represent SEM throughout.