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. Author manuscript; available in PMC: 2016 Oct 23.
Published in final edited form as: Sci Transl Med. 2015 Dec 2;7(316):316ra195. doi: 10.1126/scitranslmed.aad0966

Figure 1. Humanized, chimeric and murine antibodies have similar binding affinities to PTx.

Figure 1

A, SDS-PAGE comparing purified murine, chimeric and humanized variants of 1B7 and 11E6 in reduced and non-reduced forms as indicated. Molecular weights standards (kDa) are shown in lane 1. B, Indirect ELISA comparing the binding of all three versions of each antibody to PTx: murine (▲), chimeric ( Inline graphic) and humanized ( Inline graphic) and isotype control (×) antibodies shown. The absorbance data was normalized such that the maximum signal is 1.0; each sample was run in duplicate and each assay performed at least three times with different protein preparations.