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. Author manuscript; available in PMC: 2016 Oct 23.
Published in final edited form as: Sci Transl Med. 2015 Dec 2;7(316):316ra195. doi: 10.1126/scitranslmed.aad0966

Table 1.

Biochemical characterization of 1B7 and 11E6 antibodies

Melting temp (°C), second transition Kd, competition ELISA (nM); (# exp) Kd, SPR; nM (χ2) on-rate × 105, SPR (sec−1 M−1) off-rate × 10−4, SPR (sec−1)
m1B7 74.8 ± 0.7 0.4 ± 0.2 (5) 0.7 ± 0.2 (0.32) 1.7 ± 0.3 1.2 ± 0.3
ch1B7 78.1 ± 0.5 0.5 ± 0.3 (3) 0.5 ± 0.4 (0.74) 1.5 ± 0.1 0.8 ± 0.5
hu1B7 79.0 ± 0.3 1.2 ± 0.7 (6) 0.7 ± 0.5 (0.75) 0.9 ± 0.2 0.7 ± 0.5
m11E6 67.3 ± 0.4 5 ± 1 (3) 2.4 ± 0.9 (2.20) 1.3 ± 0.7 3 ± 3
ch11E6 69.4 ± 0.4 5 ± 2 (4) ND* ND* ND*
hu11E6 74.4 ± 0.4 7 ± 3 (5) 2.3 ± 0.7 (1.25) 0.8 ± 0.4 1.7 ± 0.7
*

ND, not determined