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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Methods. 2016 Jun 8;109:12–20. doi: 10.1016/j.ymeth.2016.06.004

Fig. 2.

Fig. 2

Expression pattern of mt-cpYFP in adult ventricular myocyte. (A) Confocal frame scan images showing typical mitochondrial morphology and organization pattern in a rat adult cardiomyocyte two days after adenovirus mediated mt-cpYFP gene transfer. TMRM (20 nM) was used as a mitochondrial marker. Scale bar = 20 µm. (B) Confocal linescan images showing laser-induced mPTP openings (sudden and irreversible loss of TMRM fluorescence) and the accompanying transient increases in mt-cpYFP fluorescence in the very early phase of mPTP opening in each mitochondria. (C) Traces showing time-dependent changes of mt-cpYFP and TMRM fluorescence in the three mitochondria (#1–3) indicated in (B). The broken lines indicate the onset of sustained membrane potential loss and the bursting and transient increase in mtcpYFP fluorescence.