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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Methods. 2016 Jun 8;109:12–20. doi: 10.1016/j.ymeth.2016.06.004

Fig. 5.

Fig. 5

Single mitochondrial flashes detected in Langendorff perfused heart. (A) Representative images of the intact myocardium showing the colocalization of mt-cpYFP and TMRM. Scale bar = 20 µm. (B) Image of a single cardiomyocyte in the intact myocardium. The area highlighted by the white boxes is enlarged to show a mitochondrial flash accompanied with decrease in membrane potential (white arrows). Scale bars = 20 and 5 µm for the full size and enlarged images, respectively. (C) Traces showing the fluorescence changes during the single mitochondrial flash highlighted in (B).