Figure 5. Increased IL-1β gene expression in 5-aza and LPS-treated primary cultured microglia coincides with decreased IL-1β promoter DNA methylation.
Primary cultured microglia were treated with 5-aza for 24 h and/or LPS for 4 h. Bars represent the mean ± SEM (n = 3–4) for A) IL-1β promoter DNA methylation as measured by a Methylation Index (MI) and B) IL-1β mRNA expression.