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. 2016 Oct 24;7:1571. doi: 10.3389/fpls.2016.01571

FIGURE 3.

FIGURE 3

HLS1 gene transcription is activated by soil cover or mechanical pressure. (A) GUS staining of 12-day-old light-grown transgenic seedlings expressing pHLS1:GUS in the Col-0 (WT) background. Seedlings grew on the plates without soil cover (No soil), were subjected to mechanical stress by the pressure of a glass plate (∼150 Pa, 6 h pressure), or emerged from 3 mm of 40-60 mesh sand particles (With soil). Scale bar = 1 mm. (B) GUS staining of 4-day-old dark-grown seedlings expressing pHLS1:GUS in the Col-0 (WT) background. Two independent pHLS1:GUS/Col-0 transgenic lines (#49-5 and #9-1) were used in the study. Etiolated seedlings were subjected to mechanical stress by the pressure of a glass plate (∼150 Pa) in the dark for the indicated time periods and then stained for GUS activity analysis. Scale bar = 1 mm.