Table 2.
Novel miRNAs regulating 11 catalytic enzymes are involved in fatty acid biosynthesis.
| miRNA | Mature sequence (5′-3′) | Size | LP | MFE | Target gene | Target annotation |
|---|---|---|---|---|---|---|
| novel_mir_104 | GAGGAAGAAGAAGAAGAAGAAGCG | 24 | 90 | −21.3 | BnaA03g37760D | Beta-ketoacyl synthase |
| novel_mir_555 | AAGGAAGAAGAAGAAGATGTAATT | 24 | 76 | −23.4 | BnaC01g40290D | fatty acyl-ACP thioesterases B (FATB) |
| novel_mir_173 | ATCTTGTCGGAGTTTATGATC | 21 | 85 | −27.2 | BnaA05g33500D | Plant stearoyl-acyl-carrier-protein desaturase family protein |
| novel_mir_19 | AGGACCTGATTGCAATGATAACGG | 24 | 90 | −18.2 | BnaA01g21430D | NAD(P)-binding Rossmann-fold superfamily protein |
| novel_mir_2311 | AGGAGAGATTGGATATCCGAACGG | 24 | 82 | −23.7 | BnaA06g13360D | fatty acid biosynthesis 1 (FAB1) |
| novel_mir_523 | AGAGGGGTTGGGGTCGGTGCG | 21 | 90 | −68.4 | BnaA09g34910D | NAD(P)-binding Rossmann-fold superfamily protein |
| novel_mir_702 | ATCGAAAACTTTGACTGATGTGCC | 24 | 84 | −20.5 | BnaC01g40290D | NAD(P)-binding Rossmann-fold superfamily protein |
| novel_mir_828 | GGATCGAATCCAGATCTCGGATA | 23 | 96 | −42.9 | BnaC03g74210D | fatA acyl-ACP thioesterase (FaTA) |
| novel_mir_2163 | AAGGGGATGATTGGTAAGTGCTGT | 24 | 94 | −38 | BnaA05g35320D | SUPER SENSITIVE TO ABA AND DROUGHT2 (SAD2) |
| novel_mir_801 | GATCGAATCCAGATCTCGGATAAA | 24 | 83 | −25.8 | BnaA03g37700D | fatA acyl-ACP thioesterase (FaTA) |
| novel_mir_298 | TTAAGAGATATAAGAACCGTCTAT | 24 | 87 | −28.2 | BnaCnng00070D | fatA acyl-ACP thioesterase (FaTA) |
| novel_mir_604 | AGAGATGGCAATCATGGACTTGGA | 24 | 97 | −27.1 | BnaC01g32050D | 3-ketoacyl-acyl carrier protein synthase I (KAS I) |
| novel_mir_113 | TTCCGTCAGAATTTCCTCGGTA | 22 | 90 | −28.7 | BnaA06g04900D | fatty acyl-ACP thioesterases B (FATB) |
| novel_mir_1407 | AGATTAGTCGGTTGGGCTTCGGCC | 24 | 87 | −20.6 | BnaA03g13780D | Malonyl CoA-acyl carrier protein transacylase |
| novel_mir_1430 | GGATCGAATCCAGATCTCGGATAA | 24 | 98 | −42.9 | BnaA03g37700D | fatA acyl-ACP thioesterase (FaTA) |
| novel_mir_1706 | GAGAGTTCGACGGCTAGGGT | 20 | 82 | −26.4 | BnaA06g06030D | acetyl Co-enzyme a carboxylase biotin carboxylase subunit (CAC2) |
| novel_mir_1728 | TCCGAACCGATCTGAACCCGACA | 23 | 97 | −21.4 | BnaA08g19070D | NAD(P)-binding Rossmann-fold superfamily protein |
| novel_mir_1823 | ATTTGGATTGGAGAAAGAGGGTAT | 24 | 100 | −19.7 | BnaC05g14920D | fatty acid biosynthesis 1 (FAB1) |
| novel_mir_1758 | GGAGGAGACGGAGGAGGAGGAGGA | 24 | 101 | −31.1 | BnaAnng14470D | Thioesterase superfamily protein |
LP, length of precursor; MFE (kcal/mol), minimal folding free energy.