Figure 2.

Effect of CsA or Spongionella compounds on plasma membrane levels of CD147 receptor in human T lymphocytes stimulated with Con A. (A) Representative cellular images and histograms of the CD147 receptor expression of untreated human T lymphocyte control cells, Con A (50 μg mL⁻¹) for 48 h treated cells and human T lymphocytes pretreated for 2 h with CsA (0.2 μM) or Spongionella compounds (1 μM) before Con A stimulation for 48 h. Brightfield images are represented in channel 4, and FITC intensity images are shown in channel 2. (B) Percentage of cells with CD147 expression in plasma membrane after Con A or CsA (0.2 μM), Gracilin H, A, L, and tetrahydroaplysulfurin-1 pretreated for 2 h with at 1 μM before Con A stimulation for 48 h. All values are presented as the percentage of fluorescence intensity and are compared to that of T lymphocytes treated with Con A 50 μg mL⁻¹ alone by ANOVA statistical analysis followed by post hoc Dunnet’s t-test. *p < 0.05, **p < 0.01, or between control cells and Con A-treated cells ^###p < 0.001. Data are the mean ± SEM of three independent experiments (10,000 cells were analyzed in each experiment).