Figure 5.

Mo-derived cells harvested from co-cultures with hAC do not trigger the allo-response of T cells. Cells derived from culture of peripheral blood Mo with GM-CSF + IL-4 (6 days, indicated as iDC) or GM-CSF + IL-4 + LPS (6 days of GM-CSF + IL-4 + 2 days of LPS, indicated as mDC) without or with hAC were harvested, extensively washed and put again in culture with allo-T cells purified from unrelated donors (T) at the T:antigen-presenting cell ratio of 10:1. The effect of unstimulated Mo [as the minimal triggering of proliferation of T cells and of CD3–CD28 mAb-coated beads (CD3-CD28 coated beads)] as the maximal T cell proliferation is shown for comparison. Results are expressed as counts of luminescence evaluating the ATP content in proliferating cell cultures and they are the mean ± SD of eight independent experiments using six different hAC preparations and eight healthy unrelated T cell donors. *p < 0.001.