Table 3.

Experimental design layout for Plackett-Burman design.

Run	A	B	C	D	E	F	G	H	I	J	K
1	8	12	1000	9	13	13	13	9	3	40	1
2	12	12	1000	9	9	9	13	9	15	80	0.2
3	12	8	1000	13	9	13	13	13	3	40	0.2
4	8	12	1000	13	9	9	9	13	3	80	1
5	8	8	200	13	9	13	13	9	15	80	1
6	8	12	200	13	13	9	13	13	15	40	0.2
7	12	8	200	9	13	9	13	13	3	80	1
8	8	8	1000	9	13	13	9	13	15	80	0.2
9	8	8	200	9	9	9	9	9	3	40	0.2
10	12	8	1000	13	13	9	9	9	15	40	1
11	12	12	200	13	13	13	9	9	3	80	0.2
12	12	12	200	9	9	13	9	13	15	40	1

A: lactose (%), B: glucose (%), C: dye concentration (rpm), D: ammonium sulphate (%), E: ammonium nitrate (%), F: tryptone (%), G: yeast (%), H: ammonium chloride (%), I: incubation time (days), J: moisture content (%), and K: inoculum size (cm).