Figure 3.

ATP treatment does not modify caspase-1 activity in Ngo-infected MDM. The fluorescent labeled probe of caspase-1 FAM-YVAD-FMK (FAM-FITC) was incubated with uninfected MDM, gonococcus-infected MDM (Ngo+MDM), or gonococcus-infected MDM and ATP (Ngo+MDM+ATP). After washing, the fluorescent intensity retained by the MDM was determined by flow cytometry. (a) Representative histograms from one assay are shown. (b) Percentage of positive FAM cells under different conditions. Data obtained are expressed as the mean ± SD and represent at least three independent experiments.